Active Ingredients: Doxycycline
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Trademarks and Approvals. You agree not to distribute, display or publish any such materials without such approval. This is a commonly used inducible model of RA, in which transient polyarthritis is evoked in healthy recipients by passive transfer of arthritogenic serum originating from a spontaneously arthritic transgenic mouse strain.
The main advantages of this model are that it is suitable for studying the B- and T-cell independent immunological mechanisms and in case of repeated serum injection the RA-associated chronic pain with neuropathic components Korganow et al.
Although the pharmacological interventions with MMP inhibitors are also valuable tools to examine the roles of MMPs, in this model their functions had only been studied using specific knockout mice.
In the present study we showed the increase of various MMP activities in the joints by in vivo fluorescence imaging together with ex vivo zymography, as well as demonstrated for the first time that SDD worsens arthritis-induced bone microarchitectural alterations most probably independently of MMP inhibition.
The total number of animals used in the experiments were 33 17 in the 30 days and 16 in the 16 days experimental series.
Induction of the Arthritis Arthritis was induced by repeated intraperitoneal i.
Since there are no data for chronic doxycycline use in arthritis, the dosage and concentration were based on earlier publications related to other issues Prall et al. To avoid the precipitate formation and reach the expected doxycycline plasma concentration, the water was acidified to pH of 3.
Mice provided with acidified tap water were used for controls. All water bottles were changed every other day by the research staff.
Experimental Design The study was performed in two series as outlined in Figure 1.
The mechanonociceptive threshold of the hind paws were evaluated on days 5, 15, and 24, neutrophil myeloperoxidase MPO activity and plasma extravasation on days 2, 14, and 21, MMP activity on day 4, changes of the periarticular bone structure on day 30, arthritis severity and joint function every day during the 30 days experimental period.