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Although the transformation was repeated a number of times, no transformant could be obtained for COV 8 harboring the blaCTX-M-1 gene, suggesting that this gene was probably borne on the chromosome, but no attempt to confirm this localization was attempted. The information on this page has been compiled for use by healthcare practitioners and consumers in the United States and therefore neither Everyday Health or its licensor warrant that uses outside of the United States are appropriate, unless specifically indicated otherwise.
Furthermore, the lack of standardized tests hinders generalizations regarding the effects of antibiotics on biogeochemical processes.
We investigated the effects of antibiotics on biogeochemical N cycling, specifically nitrification, denitrification, and anammox. We found that environmentally relevant concentrations of fluoroquinolones and sulfonamides could partially inhibit denitrification.
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This G 57 A mutation was also present in the sequence of the previously published pC 49-108 plasmid of a chicken E. Smith et al determined the pMLST of 251 IncI 1 plasmids isolated from to from humans, poultry and other animal species, and sequenced 32 of them.
The genes coding for resistance to beta-lactams blaCTX-M-1, all 30 plasmids, blaTEM-1 B, one plasmid pCOV 4, tetracyclines tet A, 20 plasmids, sulphonamides sul 2, 27 plasmids, trimethoprim dfrA 1, one plasmid, dfrA 17, five plasmids and aminoglycosides aadA 5, five plasmids were detected and were in agreement with the resistance profiles of the transformants except for pCOV 19, for which no tetracycline-resistance gene could be detected.
It is worthy of note that the resistance genes that are most often carried on the blaCTX-M plasmid are resistances to tetracyclines and trimethoprime-sulphonamides, antimicrobials that are frequently used in poultry production.
They also shared the genes involved in maintenance and stability regions. Similarly, the iss gene had been detected on seven out of eight commensal strains and on 14 out of 14 pathogenic ones.
A putative transposase of 1181 bp was present in 22 plasmids and was identical to the transposase previously reported in other plasmids such as pC 49-108 accession number KJ, region: — or p 369 accession number KT, region: 87786—88966.
This transposase was found to be located at 19—22 kbp from the blaCTX-M-1 gene.
The 806 bp IS 26 was present on 26 plasmids at approximately 24.